Technology Transfer Office

Antibodies

SD2007-014

Zebrafish Model for Atherosclerosis

UC San Diego researchers have developed a novel zebrafish model useful for studying mechanisms of accumulation of lipid in blood vessel walls and associated vascular inflammation.

This invention describes synthesis, compositions, and methods related to the use of fluorescent nucleoside analogs as probes for nucleic-acid structure, dynamics, and function, as well as sequence and lesion analysis.

Related Materials:

For anti-giantin: Lesa GM, Seemann J, Shorter J, Vandekerckhove J, Warren G. The amino-terminal domain of the golgi protein giantin interacts directly with the vesicle-tethering protein p115.
J Biol Chem. 2000 Jan 28;275(4):2831-6.

For anti-Golgi integral membrane protein: Stanley H, Botas J, Malhotra V. The mechanism of Golgi segregation during mitosis is cell type-specific.
Proc Natl Acad Sci U S A. 1997 Dec 23;94(26):14467-70.

Related Materials: Tyler JK, Collins KA, Prasad-Sinha J, Amiott E, Bulger M, Harte PJ, Kobayashi R, Kadonaga JT. Interaction between the Drosophila CAF-1 and ASF1 chromatin assembly factors. Mol Cell Biol. 2001 Oct;21(19):6574-84.

Reagents to Block Cell Division

SD2001-224

and

SD2001-225

Monoclonal and Polyclonal Antibodies Directed Against Myosin Motors— Useful for the Study of Deafness

Both monoclonal antibodies (SD2001-224)and polyclonal antibodies (SD2001-225) have been prepared which bind to the unconventional myosin, myosin VIIa, an actin-bindin protein, which has been shown to be involved in auditory signal transmission and may be useful for the biochemical and immunohistochemical research on deafness, blindness, and phagocytic events.

SD2001-093

LJ-26, A Recombinant Chicken Monoclonal Antibody with Defined Molecular Specificity for Antibody VH Regions

Recombinant avian antibody obtained by phage display technology. (See reference.)

Kinesin proteins are used by neurons for transport along microtubules. The A protein is found throughout neurons while the B protein is enriched in dendrites. Antibodies were raised against these two newly discovered kinesins with amino acid similarity, but different localizations. The antibodies are affinity purified and were raised again fusion proteins containing amino acids 1124-1355 (A) and 1135-1419 (B). (See reference.)

SD1999-129

Polyclonal Antibodies to IRF3

Polyclonal antibodies to IRF3 have been prepared by immunizing rabbits with full-length human IRF3 fused to glutathione S-transferase. These antibodies are suitable for use in immunoprecipitations, western blotting, immunofluorescence and supershift assays. Reactive with human and bovine IRF3, weakly cross-reactive with murine IRF3.

Related Materials: Navarro, L. and David, M. Journal of Biological Chemistry Vol. 274, No. 50 (1999), 35535-38.

Polyclonal antibodies to STAT1 have been prepared in rabbits and are suitable for use in immunoassays.

Monoclonal Antibodies to PP2A

Inquiries To: invent@ucsd.edu

Polyclonal Sera to Deformed Homeobox Proteins

Highly specific polyclonal antibody preparations are available that target kinesin molecular motors from a number of sources. These can be used for immunostaining, or functional inactivation of molecular motor function. The preparations are available as non-patented tangible research materials under an appropriate material transfer agreement for non-commercial purposes, or for resale as laboratory reagents under a non-exclusive bailment license.

Monoclonal Antibodies to Anti-Rat Alcohol Dehydrogenase

Description

UC San Diego investigators have improved a version of the reverse two hybrid screen designed for the purpose of screening large chemical libraries of inhibitors of a given protein-protein interaction. They have created a small molecule permeable reporter yeast strain, a positive control activator and a panel of selective inhibitors which serve as positive controls. These improvements may significantly increase the probability of identifying a small molecule inhibitor of any protein-protein interaction that can be detected by two-hybrid analysis.

Chen L, Widhopf G, Huynh L, Rassenti L, Rai KR, Weiss A, Kipps TJ. Expression of ZAP-70 is associated with increased B-cell receptor signaling in chronic lymphocytic leukemia. Blood. 2002 Dec 15;100(13):4609-14.

Related Materials: Viriyakosol, S., Tobias, P.S., Kitchens, R.L. and Kirkland., T.N. (2001) J.Biol.Chem. 276: 38044-38051

Presenilin-1 is required for the production of beta-amyloid. The homozygous deficient and the hemizygous knock-out companion cell lines comprise a family of cell lines to study the biology of the presenilin protein and the production pathways of amyloid precursor protein and beta-amyloid protein. Four immortalized cell lines are available:

• - / - (PS1 Deficient)
• + / - (PS1)
• + / - (PS2)
• + / - (APP)
                                                                  

(See reference.)

Caveolin-3 is one of the proteins that regulate signaling pathways and is thought to function in cardiac protection (ischemic preconditioning). Associated studies indicate that the novel caveolin-3 over-expressing mouse is resistant to myocardial ischemia/reperfusion injury, suggesting that caveolin-3 as a potential therapeutic for patients undergoing cardiac surgery or at high risk for myocardial infarction.

This invention is  a transgenic mouse which carries the entire full-length human CYP1A1 gene plus the regulatory or flanking DNA. When the animals are exposed to certain environmental toxicants, the gene is induced through activation of the Aryl hydrocarbon (Ah) or dioxin receptor. Detection of gene expression can then be demonstrated by usual laboratory techniques such as enzymatic expression or Western blot. These mice are unique in that an entire human CYP1A1 gene is in a mouse.

Suggested Uses: 

1. These mice can be used to identify agents that induce the gene or activate the transcriptional regulator of the gene, i.e. toxicants.
2. To identify drugs that are potential targets for the Ah receptor.
3. An excellent model system to identify environmental toxicants.
4. Can be used to "humanize" mice for the CYP1A1 gene.
5. Can be used to explore the developmental impact of agents that might induce the CYP1A1 gene during development.
6. Can be used to understand signal transduction and events controlling the Ah receptor.

Also see SD2004-144, for transgenic mice containing  the CYP1A1 gene linked to a luciferase reporter gene.

Transgenic mice expressing hBACEI have been generated and characterized behaviorally.

This transgenic mouse model exists in several lines, each demonstrating an extensive neurological phenotype at early stages of development. Reversal of the phenotype with a known inhibitor has already been shown thus confirming the utility of this model for drug screening and validation.

Reference: SD2003-069-JBC

Collagen-induced arthritis has been used for many years as a model to evaluate potential therapeutic agents in arthritis. We have developed a strain of mice with more severe arthritis than standard collagen-induced arthritis. This strain has defective apoptosis in the joints and increased production of proteases and cytokines.

Suggested Uses: This mouse model will be useful for identifying and developing therapeutic agents that induce apoptosis in arthritis, thereby overcoming the defect. In addition, it provides a more stringent test of anti-inflammatory agents and protease inhibitors because the disease is more severe and destructive than in presently available strains.

UC San Diego researchers have constructed transgenic mice with a germline deletion of the mouse macrophage galactose/N-acetylgalactosamine-specific C-type lectiin (mMGL). This lectin is normally expressed in monocytes, macrophages, and dendritic cells.The deletion is maintained in C57BL/6 mice.  These transgenic animals are useful for research on tumoricidal activity and innate immunity to certain pathogens. (See reference.)

Adenovirus vector, subtype 5 (Ad5) encoding human CD40. See publication below for additional details.

Bashey A, Cantwell MJ, Kipps TJ. Adenovirus transduction to effect CD40 signalling improves the immune stimulatory activity of myeloma cells. Br J Haematol. 2002 Aug;118(2):506-13.

Applications:

Agriculture companies may be interested in obtaining the plasmid for genetic manipulation of their plants to enhance leaf greening and growth, and regulation of stomatal opening and closure. In addition, basic researchers might want antibodies made against the specific plant nitric oxide synthase.

Genomic DNA from Bugula Neritina and Its Symbiont "Candiadtus Endobugula Sertula"

Applications: As a research tool in organisms from yeast to mice, or in cell lines, this method may have a modulating effect upon gene expression rather than a complete “on or off” switch. The present approach allows multiple discrete allele strengths to be obtained from a single transgene conditional upon a second unlinked transgene, a chromosomal locus or an expressed protein.

These transgenes can be used in the generation of transgenic mice to direct macrophage specific expression of foreign genes. (See reference.)

SD2000-076

Pcre-Hygro Plasmid

*Use of this plasmid to generate transgenic mice requires the recipient to have a license with DuPont.

*Use of this plasmid to generate transgenic mice requires the recipient to have a license with DuPont.

VEGF DNA was isolated from sheep placenta and various fetal tissues. A 164 amino acid peptide was the dominant form, with lower level of expression found of the 120 and 188 amino acid peptides. The VEGF₁₆₄ signal peptide sequence is highly conserved.  This cDNA is useful as a probe in Northern/Southern hybridization studies and for localization of VEGF mRNA in ovine tissues or cells. It can also be used in transfection experiments. (See reference.)

A UC San Diego researcher, along with researchers from sister academic institutions, have isolated from a caveolae-specific glycoprotein, named PV-1 and a cDNA encoding it from mouse and rat endothelial cells. PV-1 is an integral membrane protein of caveolae and appears to be expressed mostly in the lung, with little or no expression observed in the kidney, spleen, liver, heart, muscle and brain. PV-1 would be useful as a target for lung-specific drug delivery.

BAG1 and Its Homolog from Brassicaeae; Useful for Manipulation of Flower Structure

This vector offers increased frequency of functional inserts while minimizing the frequency of defective inserts because stop codons in the cDNA do not interfere with phage display. Cloning into this vector results in a c-terminal fusion product with the Fos protein and helper phage infection induces expression of the fusion product and the Jun-gpIII product. Selection is based on ampicillin resistance and expression is inducibile by the lac promotor. (See reference.)

Although sodium channels often provide targets for various classes of drugs, studies of their molecular structure and specific pharmacology have been limited by the availability of tissue. Two full length cDNA (HBA AND HBB) clones specific for the sodium channel alpha subunit were isolated from cerebral cortex. The HBA clone has been expressed in CHO cells. These cDNA clones provide a new tool for the development of additional therapeutic compounds. (See reference.)

Novel cDNAs taken from gastric tissue have been cloned and various products of the UDP-Glucuronosyltransferase gene expressed in a baculovirus system. These proteins are useful in the investigation of drug metabolism. They also have potential application in gene therapy studies for the treatment of the lethal negative mutation of the enzyme characteristic of Crigler-Najjar syndrome. (See reference.)

UC San Diego researchers have developed a novel adenovirus vector which is useful for in vivo tissue-specific (e.g., cardiac muscle) expression of a transgene.

Advantages for this technology are:  
Efficient—provides high gene delivery.
Specific—provides tissue-specific expression of a transgene.  
Broad Application—applicable to other tissue types.

Selected territories available.

Homeobox proteins are homeodomain-containing transcription factors which differentially regulate downstream gene expression important in morphological diversification. This antibody was raised against a full length recombinant Drosophila Dfd protein. The gene that encodes this protein is necessary for the development of the maxillary and mandibular segments of the fly head. (See reference.)

Tyrosine Kinase Produced from EGF

Inquiries To: invent@ucsd.edu

*Use of this plasmid to generate transgenic mice requires the recipient to have a license with DuPont.

SD2004-A39

Protecting groups can be used to mask compounds, or portions of compounds, from interacting in chemical or biological systems.

Technology Description: The invention relates to protecting groups that can be removed by irradiation. In general, the invention features a protecting group derived from a halogenated coumarin group, a quinoline-2-one group, a xanthene group, a thioxanthene group, a selenoxanthene group, or an anthracene group. The protecting group is photolabile and can be removed by irradiating the group with light, such as flash photolysis with ultraviolet radiation or pulsed infrared radiation.

Advantages:
• Using light to control the photoactivation, the invention provides an outstanding temporal precision that enables diverse applications such as photolithographic construction of complex peptide and oligonucleotide arrays.
• A valuable strategy for in vivo biological applications, allowing control of the onset of bioactivity in living cells with millisecond temporal precision.

Blood Sample Library of Rare Leukemia Forms

Technology Description: A glucuronic C5 epimerase suitable for use in production chemistry of complex polysaccharides has been sequenced and made available through its recombinant form. Cells transformed with the cDNA for this C5 epimerase are able to convert D-glucuronic acid to L-iduronic acid. Heparin and similar molecules in the cell are modified to iduronioc acid analogs, yielding modified glycans with differing properties.

Advantages: The C-5 transformation of heparin and related molecules has not previously been practical to conduct in practical scale.

State of Development: Preclinical

Patent Information: Pending

Inquiries to: invent@ucsd.edu

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